The BJP ad mocks Mr Kejriwal for taking the support of the Congress to form government after the last election in December, 2013, and seems to imply that the AAP chief went against the principles of his former mentor Anna Hazare, using a garlanded portrait of the Gandhian.
"Nathuram Godse killed Gandhiji on this day in 1948. BJP has killed Anna in its ad today. Shudn't BJP apologise?" Arvind Kejriwal said in a tweet.
Earlier this week, Anna Hazare said he was "not interested" in talking about Arvind Kejriwal and BJP's presumptive chief minister Kiran Bedi, who are now main rivals in the fight for Delhi.
He was responding to NDTV's questions about gathering a new team for his campaign against Prime Minister Narendra Modi, after the break-up of "team Anna", which was at the core of his massive anti-corruption campaign in 2011. Arvind Kejriwal and Kiran Bedi were the two most prominent faces of his old team.
"Ekla Chalo Re. I don't need Kiran Bedi or Arvind Kejriwal. I have the public with me," said the 77-year-old social activist.
In the last election, Anna Hazare, who had been openly disapproving of Mr Kejriwal's entry into politics, had banned AAP from using his name or images in their campaign.
Delhi votes for a new government on February 7. The results will be declared on February 10.